immpact novared detection reagent Search Results


immpact novared peroxidase (hrp) substrate  (Vector Laboratories)
95
Vector Laboratories immpact novared peroxidase (hrp) substrate
Immpact Novared Peroxidase (Hrp) Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector immpact novared peroxidase substrate kit  (Vector Laboratories)
96
Vector Laboratories vector immpact novared peroxidase substrate kit
Vector Immpact Novared Peroxidase Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dab peroxidase substrate  (Vector Laboratories)
96
Vector Laboratories dab peroxidase substrate
Dab Peroxidase Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immpact novared  (Vector Laboratories)
86
Vector Laboratories immpact novared
Immpact Novared, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain abc elite kit  (Vector Laboratories)
99
Vector Laboratories vectastain abc elite kit
Vectastain Abc Elite Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immpact novared® substrate  (IMMPACT Biotechnologies GmbH)
90
IMMPACT Biotechnologies GmbH immpact novared® substrate
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Immpact Novared® Substrate, supplied by IMMPACT Biotechnologies GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immpact novared® substrate - by Bioz Stars, 2026-03
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avidinbiotin-peroxidase method  (Vector Laboratories)
94
Vector Laboratories avidinbiotin-peroxidase method
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Avidinbiotin Peroxidase Method, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hrp  (Vector Laboratories)
86
Vector Laboratories hrp
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Hrp, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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novared  (Vector Laboratories)
86
Vector Laboratories novared
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Novared, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immpact novared chromogen  (Vector Laboratories)
86
Vector Laboratories immpact novared chromogen
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Immpact Novared Chromogen, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immpact novared chromogen - by Bioz Stars, 2026-03
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aec hrp substrate  (Vector Laboratories)
93
Vector Laboratories aec hrp substrate
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Aec Hrp Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , f IHC staining of substantia nigra of brain from WT mice using ImmPACT NovaRED ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.

Journal: NPJ Parkinson's Disease

Article Title: CRBN modulates synuclein fibrillation via degradation of DNAJB1 in mouse model of Parkinson disease

doi: 10.1038/s41531-024-00801-3

Figure Lengend Snippet: a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , f IHC staining of substantia nigra of brain from WT mice using ImmPACT NovaRED ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.

Article Snippet: CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , f IHC staining of substantia nigra of brain from WT mice using ImmPACT NovaRED ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining.

Techniques: Transfection, Incubation, Injection, Immunohistochemistry, Staining, Gentle

Reagents and peptides used in the study

Journal: NPJ Parkinson's Disease

Article Title: CRBN modulates synuclein fibrillation via degradation of DNAJB1 in mouse model of Parkinson disease

doi: 10.1038/s41531-024-00801-3

Figure Lengend Snippet: Reagents and peptides used in the study

Article Snippet: CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , f IHC staining of substantia nigra of brain from WT mice using ImmPACT NovaRED ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining.

Techniques: Protease Inhibitor, Plasmid Preparation, Recombinant